Rat leptin (LEP) ELISA kit (0.1-20 ng/mL)

Cat. No.: 0126WXX-833

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LEP

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Rat leptin (LEP) ELISA kit (0.1-20 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat leptin (LEP) ELISA kit (0.1-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in rat samples with a range of 0.1-20 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is a sandwich ELISA featuring a microplate pre-coated with a rat LEP capture antibody. Rat LEP in the sample binds to the immobilized antibody, followed by the binding of a biotinylated rat LEP detection antibody and streptavidin-HRP. After incubation and a washing step, a substrate solution is introduced to produce color in direct proportion to the amount of rat LEP. The reaction is stopped with an acidic solution and measured at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Rat leptin (LEP) ELISA kit (0.1-20 ng/mL) is used to quantify LEP in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of rat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.1-20 ng/mL

Sensitivity: 0.05 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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