Mouse fatty acid synthase (FAS) ELISA kit (0.156-10 ng/mL, 0.057 ng/mL)

Cat. No.: OB0625WXX-865

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: FAS

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Mouse fatty acid synthase (FAS) ELISA kit (0.156-10 ng/mL, 0.057 ng/mL)

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Product Overview

Description: Mouse fatty acid synthase (FAS) ELISA kit (0.156-10 ng/mL, 0.057 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in mouse with a range of 0.156-10 ng/mL and a specificity of 0.057 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with FAS-specific antibodies. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated FAS-specific antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. Only wells containing FAS, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of FAS in the sample can then be determined by comparing the OD value of the sample to a standard curve.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Mouse fatty acid synthase (FAS) ELISA kit (0.156-10 ng/mL, 0.057 ng/mL) is used to quantify FAS in tissue homogenates, cell lysates, and other biological fluid samples of mouse, providing data to support a wide range of studies.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.057 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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