Mouse butyrylcholinesterase (BCHE) ELISA kit (160-100000 pg/mL, <59 ng/mL)

Cat. No.: OB0625WXX-234

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: BCHE

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Mouse butyrylcholinesterase (BCHE) ELISA kit (160-100000 pg/mL, <59 ng/mL)

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Product Overview

Description: Mouse butyrylcholinesterase (BCHE) ELISA kit (160-100000 pg/mL, <59 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of BCHE in mouse with a range of 160-100000 pg/mL and a specificity of <59 ng/mL.

Assay Principle: BCHE-specific antibody has been pre-coated on microtiter plates. Biotin-labeled BCHE and unlabeled BCHE (standards or samples) undergo a competitive inhibitory reaction with the pre-coated BCHE-specific antibody. After incubation, unbound conjugates are washed away. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. The amount of bound HRP is inversely proportional to the concentration of BCHE in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of BCHE in the sample.

Background: BCHE is a non-specific cholinesterase that has broad substrate specificity and is involved in detoxification reactions, drug metabolism, among other processes.

Synonyms: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; EC 3.1.1.8

EC NO.: 3.1.1.8

Applications: Mouse butyrylcholinesterase (BCHE) ELISA kit (160-100000 pg/mL, <59 ng/mL) is used to quantify BCHE in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of mouse, providing data to support a wide range of studies.

Research Area: Metabolic pathway

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 160-100000 pg/mL

Sensitivity: <59 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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