Rat butyrylcholinesterase (BCHE) ELISA kit (1.56-100 ng/mL, 0.58 ng/mL)

Cat. No.: OB0625WXX-236

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: BCHE

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Rat butyrylcholinesterase (BCHE) ELISA kit (1.56-100 ng/mL, 0.58 ng/mL)

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Product Overview

Description: Rat butyrylcholinesterase (BCHE) ELISA kit (1.56-100 ng/mL, 0.58 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of BCHE in rat with a range of 1.56-100 ng/mL and a specificity of 0.58 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for BCHE. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for BCHE. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. Only wells containing BCHE, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of BCHE in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: BCHE is a non-specific cholinesterase that has broad substrate specificity and is involved in detoxification reactions, drug metabolism, among other processes.

Synonyms: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; EC 3.1.1.8

EC NO.: 3.1.1.8

Applications: Rat butyrylcholinesterase (BCHE) ELISA kit (1.56-100 ng/mL, 0.58 ng/mL) is used to quantify BCHE in serum, plasma, tissue homogenates, and other biological fluid samples of rats, providing data to support a wide range of studies.

Research Area: Metabolic pathway

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Other biological fluids

Detection Range: 1.56-100 ng/mL

Sensitivity: 0.58 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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