Rat insulin (INS) ELISA kit (140.62-9000 pg/mL)

Cat. No.: 0126WXX-619

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: INS

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Rat insulin (INS) ELISA kit (140.62-9000 pg/mL)

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Product Overview

Description: Rat insulin (INS) ELISA kit (140.62-9000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in rat samples with a range of 140.62-9000 pg/mL and a minimum detectable dose (sensitivity) of 37.3 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to INS is pre-coated onto a microplate, and a competitive reaction is launched between biotin-labeled INS and unlabeled INS (from standards or samples) for the limited antibody binding sites. After incubation and washing to remove unbound conjugates, avidin-conjugated HRP is added to each well. Upon the addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of INS present in the sample.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Rat insulin (INS) ELISA kit (140.62-9000 pg/mL) is used to quantify INS in serum, plasma, tissue homogenates, and cell lysates, cell culture supernatants, and other biological fluids of rat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 140.62-9000 pg/mL

Sensitivity: 37.3 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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