Rat apolipoprotein C-II (APOC2) ELISA kit (0.312-20 ng/mL)

Cat. No.: OB0625WXX-128

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: APOC2

Size:

Rat apolipoprotein C-II (APOC2) ELISA kit (0.312-20 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Rat apolipoprotein C-II (APOC2) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of APOC2 in rats with a range of 0.312-20 ng/mL and a specificity of 0.137 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an antibody specific for APOC2. Standards or samples are then added to the corresponding microtiter plate wells along with a biotin-conjugated antibody specific for APOC2. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only wells containing APOC2, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of APOC2 in the sample.

Background: Apolipoprotein C-II is a component of very low-density lipoproteins and chylomicrons. It activates lipoprotein lipase and plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.

Synonyms: Apolipoprotein C2; APC2; APO-CII; APOC-II; Apo-C2; ProapoC-II; Proapolipoprotein C-II

Applications: Rat apolipoprotein C-II (APOC2) ELISA kit (0.312-20 ng/mL) is used to quantify APOC2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernatant, and other biological fluid samples of rat, providing data to support a wide range of studies.

Research Area: Metabolic pathway; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.137 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out