Rat high density lipoprotein (HDL) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-350

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: HDL

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Rat high density lipoprotein (HDL) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat high density lipoprotein (HDL) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HDL in rat samples with a range of 3.125-200 ng/mL and a minimum detectable dose (sensitivity) of 1.875ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs the sandwich-ELISA technique to measure rat HDL. The micro ELISA plate is pre-immobilized with a specific antibody. Standards or samples are added to the wells and captured by the antibody. A biotinylated detection antibody and avidin-HRP conjugate are added sequentially and incubated. After a washing step, a substrate solution is added to each well. Wells containing the rat HDL sandwich complex will turn blue and then yellow after the addition of the stop solution. The OD is measured at 450 nm ± 2 nm, allowing the concentration of rat HDL to be calculated against a standard curve.

Background: HDL is a lipoprotein that transports cholesterol from body tissues—including arterial walls—back to the liver for processing or excretion. Due to its unique “reverse cholesterol transport” function, HDL is commonly referred to as “good cholesterol.” It helps clear excess cholesterol, preventing its accumulation on blood vessel walls and thereby reducing the risk of atherosclerosis and cardiovascular disease. A close and significant relationship exists between HDL levels and obesity. Obesity, particularly an increase in abdominal fat (visceral fat), is a primary cause of decreased HDL levels.

Synonyms: HDL-C

Applications: Rat high density lipoprotein (HDL) ELISA kit is used to quantify HDL in serum, plasma, and biological fluids of rat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, hepatology, nutrition metabolism, obesity, and others.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 3.125-200 ng/mL

Sensitivity: 1.875ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 0.91

Storage: Store at 4°C.

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