Rabbit insulin (INS) ELISA kit (5.0-100 μIU/mL)

Cat. No.: 0126WXX-614

Assay Type: Quantitative sandwich ELISA

Target Species: Rabbit

Assay Target: INS

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Rabbit insulin (INS) ELISA kit (5.0-100 μIU/mL)

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Product Overview

Description: Rabbit insulin (INS) ELISA kit (5.0-100 μIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in rabbit samples with a range of 5.0-100 μIU/mL and a minimum detectable dose (sensitivity) of 1.0 μIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This INS ELISA Kit utilizes a competitive enzyme immunoassay technique. Endogenous insulin in the sample competes with a fixed amount of HRP-conjugated INS for a limited number of binding sites on a microplate pre-coated with a monoclonal anti-INS antibody. Following a one-hour incubation and washing, an HRP substrate is added to generate a blue product that turns yellow upon acidification. The optical density at 450 nm is inversely proportional to the INS concentration, which is then interpolated from a standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Rabbit insulin (INS) ELISA kit (5.0-100 μIU/mL) is used to quantify INS in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of rabbit, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 5.0-100 μIU/mL

Sensitivity: 1.0 μIU/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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