Porcine glucagon (GCG) ELISA kit (2.5-50 ng/mL)

Cat. No.: 0126WXX-74

Assay Type: Quantitative ELISA

Target Species: Porcine

Assay Target: GCG

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Porcine glucagon (GCG) ELISA kit (2.5-50 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Porcine glucagon (GCG) ELISA kit (2.5-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in porcine samples with a range of 2.5-50 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: A monoclonal capture antibody specific for GCG is pre-coated onto the microtiter plate. Standards or samples are added to the wells to allow GCG capture. To quantify the analyte, a standardized preparation of HRP-conjugated polyclonal antibody is added to each well to form a sandwich complex. After incubation, the wells are thoroughly washed. A substrate solution is introduced, and the HRP enzyme reacts to produce a color signal directly proportional to the amount of GCG bound. The reaction is terminated by sulfuric acid, and the color change is measured at 450 nm.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Porcine glucagon (GCG) ELISA kit (2.5-50 ng/mL) is used to quantify GCG in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of porcine, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 2.5-50 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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