Mouse uncoupling protein-1 (UCP1) ELISA kit (78-5000 pg/mL)

Cat. No.: 0126WXX-2042

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: UCP1

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Mouse uncoupling protein-1 (UCP1) ELISA kit (78-5000 pg/mL)

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Product Overview

Description: Mouse uncoupling protein-1 (UCP1) ELISA kit (78-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP1 in mouse samples with a range of 78-5000 pg/mL and a minimum detectable dose (sensitivity) of <38 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to the target antigen. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for the target antigen. Subsequently, avidin conjugated to HRP is added to each microplate well and incubated. A TMB substrate solution is then added to each well. Only those wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the target antigen is determined by comparing the O.D. of the samples to the standard curve.

Background: Uncoupling protein 1 (UCP1) is a mitochondrial transporter that mediates proton leakage across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis and dissipating energy as heat. UCP1 plays a key role in brown adipose tissue thermogenesis and energy expenditure, contributing to the regulation of whole-body metabolism. Impaired UCP1 function can reduce thermogenic capacity and promote energy storage, linking it to obesity and related metabolic disorders.

Synonyms: SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; Uncoupling Protein 1; Mitochondrial; Mitochondrial brown fat uncoupling protein 1

Applications: Mouse uncoupling protein-1 (UCP1) ELISA kit (78-5000 pg/mL) is used to quantify UCP1 in serum, plasma, tissue homogenates, cell culture supernatant, and other biological fluids of mice, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernatants; Other biological fluids

Detection Range: 78-5000 pg/mL

Sensitivity: <38 pg/mL

Precision (Intra-assay): CV≤4.7%

Precision (Inter-assay): CV≤8.6%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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