Rat tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L)

Cat. No.: 0126WXX-1999

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: TRACP 5b

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Rat tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L)

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Product Overview

Description: Rat tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRACP 5b in rat samples with a range of 0.78-50 U/L and a minimum detectable dose (sensitivity) of <0.08 U/L. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rat TRACP-5b monoclonal antibody, and the detecting agent is a biotin-labeled polyclonal antibody. Samples and the biotin-labeled antibody are added to the ELISA plate wells and washed with PBS or TBS. Avidin-peroxidase conjugates are then added in order. After the reactants are thoroughly washed away, the TMB substrate is used for visualization. TMB turns blue through peroxidase catalysis and eventually yellow under the action of acid. The color depth is positively correlated with the concentration of the target analyte in the sample.

Background: TRACP 5B, a metallophosphoesterase located at 19p13.2, encodes a tartrate-resistant acid phosphatase (EC 3.1.3.2) essential for bone resorption and the negative regulation of inflammatory cytokines, including TNF and interleukin-1 beta. By dephosphorylating osteopontin and bone sialoprotein, it maintains skeletal integrity and immune homeostasis; consequently, inactivating mutations lead to SPENCDI, while its elevated expression serves as a biomarker for various leukemias and Gaucher disease. In the context of metabolic health, TRACP 5B is increasingly recognized for its role in obesity-driven chronic inflammation, as its regulation of osteopontin signaling and macrophage activity directly influences the adipose tissue remodeling and systemic insulin resistance associated with excessive weight gain.

Synonyms: ACP5; TRAP5b; SPENCDI; TRACP-5b

Applications: Rat tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L) is used to quantify TRACP 5b in rat serum, plasma, cell culture supernatant, and other sources, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, and others.

Research Area: Signal transduction; Metabolism; Obesity

Specification

Sample Type: Rat serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.78-50 U/L

Sensitivity: <0.08 U/L

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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