Mouse tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L)

Cat. No.: 0126WXX-1989

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: TRACP 5b

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Mouse tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L)

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Product Overview

Description: Mouse tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRACP 5b in mouse samples with a range of 0.78-50 U/L and a minimum detectable dose (sensitivity) of <0.1 U/L. The kit is highly sensitive and easy to use.

Assay Principle: This kit applies the double-antibody sandwich ELISA method, using a microplate pre-coated with an anti-mouse TRACP-5b monoclonal antibody. A biotinylated polyclonal antibody is utilized as the detection agent. Samples and biotinylated antibodies are incubated in the wells and washed with PBS or TBS. Subsequently, avidin-peroxidase conjugates are added. After a comprehensive wash step, TMB substrate is introduced for coloration. Peroxidase activity transforms the TMB into a blue product, which turns yellow after the addition of the stop solution (color reagent C). The color intensity is positively correlated with the quantity of target analyte present in the sample.

Background: TRACP 5B, a metallophosphoesterase located at 19p13.2, encodes a tartrate-resistant acid phosphatase (EC 3.1.3.2) essential for bone resorption and the negative regulation of inflammatory cytokines, including TNF and interleukin-1 beta. By dephosphorylating osteopontin and bone sialoprotein, it maintains skeletal integrity and immune homeostasis; consequently, inactivating mutations lead to SPENCDI, while its elevated expression serves as a biomarker for various leukemias and Gaucher disease. In the context of metabolic health, TRACP 5B is increasingly recognized for its role in obesity-driven chronic inflammation, as its regulation of osteopontin signaling and macrophage activity directly influences the adipose tissue remodeling and systemic insulin resistance associated with excessive weight gain.

Synonyms: ACP5; TRAP5b; SPENCDI; TRACP-5b

Applications: Mouse tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.78-50 U/L) is used to quantify TRACP 5b in serum, plasma, and cell culture supernatant of mice, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, and others.

Research Area: Signal transduction; Metabolism; Obesity