Mouse pro-opiomelanocortin (POMC) ELISA kit (0.391-25 ng/mL)

Cat. No.: 0126WXX-1443

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: POMC

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Mouse pro-opiomelanocortin (POMC) ELISA kit (0.391-25 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse pro-opiomelanocortin (POMC) ELISA kit (0.391-25 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in mouse samples with a range of 0.391-25 ng/mL and a minimum detectable dose (sensitivity) of 0.234 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-mouse POMC antibody is pre-coated onto 96-well plates. Standards, samples, and a biotin-conjugated anti-mouse POMC detection antibody are added to the wells and washed. Subsequently, HRP-streptavidin is introduced, and unbound conjugates are washed away. TMB substrate is used to visualize the enzymatic reaction, producing a yellow color upon acidification. The density of the yellow signal is proportional to the concentration of mouse POMC captured in the plate, measured at 450 nm.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Mouse pro-opiomelanocortin (POMC) ELISA kit (0.391-25 ng/mL) is used to quantify POMC in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.391-25 ng/mL

Sensitivity: 0.234 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 88-105%

Storage: Store at -20°C (microplate; standard) and 2-8°C (other reagents).

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