Mouse cathepsin S (CTSS) ELISA kit (31.25-2000 pg/mL, <7.8 pg/mL)

Cat. No.: OB0625WXX-535

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: CTSS

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Mouse cathepsin S (CTSS) ELISA kit (31.25-2000 pg/mL, <7.8 pg/mL)

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Product Overview

Description: Mouse cathepsin S (CTSS) ELISA kit (31.25-2000 pg/mL, <7.8 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSS in mouse with a range of 31.25-2000 pg/mL and a specificity of <7.8 pg/mL.

Assay Principle: Antibodies specific for CTSS have been pre-coated on microtiter plates. Standards and samples are pipetted into the wells, and any CTSS is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for CTSS is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of CTSS bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Background: CTSS is an important member of the cysteine protease family. As a potent mammalian elastase, it can degrade various extracellular components, including fibronectin, laminin, elastin, and collagen. CTSS is abnormally expressed in various disease states, such as obesity and atherosclerosis, which makes it a biomarker and a potential therapeutic target. Studies have found that CTSS inhibitors may be a possible strategy for treating obesity.

Synonyms: CTS-S; EC 3.4.22.27

EC NO.: 3.4.22.27

Applications: Mouse cathepsin S (CTSS) ELISA kit (31.25-2000 pg/mL, <7.8 pg/mL) is used to quantify CTSS in serum, plasma, tissue homogenates, and cell lysate samples of mouse, providing data to support a wide range of studies.

Research Area: Obesity research; Immune response; Signaling pathway; Protein degradation

Specification

Sample Type: Serum; Plasma; Tissue homogenates and Cell lysates

Detection Range: 31.25-2000 pg/mL

Sensitivity: <7.8 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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