Mouse adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL, 4.94 pg/mL)

Cat. No.: OB0525WXX-184

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: ACTH

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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL, 4.94 pg/mL)

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Product Overview

Description: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL, 4.94 pg/mL) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 12.35-1000 pg/mL and a sensitivity of 4.94 pg/mL.

Assay Principle: The assay utilizes a competitive inhibitor enzyme immunoassay technique. Antibodies specific for ACTH are pre-coated on microtiter plates. Biotin-labeled ACTH and unlabeled ACTH (standards or samples) undergo a competitive inhibition reaction with the pre-coated ACTH-specific antibody. After incubation, unbound conjugates are washed away. Next, avidin bound to HRP is added to each microtiter plate well and incubated. The amount of bound HRP is inversely proportional to the concentration of ACTH in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of ACTH in the sample.

Background: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.

Synonyms: ACTH; Corticotropin; Adrenocorticotropin; Adrenocorticotropic hormone; Pomc1; Pomc-1; Beta-LPH; AlphaMSH; beta-MSH; Gamma-LPH; Alpha-MSH; Gamma-MSH

Applications: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL, 4.94 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, and other biological fluid samples.

Research Area: Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 12.35-1000 pg/mL

Sensitivity: 4.94 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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