Mouse insulin (INS) ELISA kit (1.56-100 mIU/L)

Cat. No.: 0126WXX-594

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: INS

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Mouse insulin (INS) ELISA kit (1.56-100 mIU/L)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse insulin (INS) ELISA kit (1.56-100 mIU/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in mice samples with a range of 1.56-100 mIU/L and a minimum detectable dose (sensitivity) of 0.6 mIU/L. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a double-antibody sandwich ELISA technique using a pre-coated mouse INS monoclonal antibody and a biotin-labeled polyclonal detection antibody. Samples and detection antibodies are incubated in the plate and washed with PBS or TBS before avidin-peroxidase conjugates are added. Following a final wash, TMB substrate facilitates color development, which shifts from blue to yellow under acidic conditions. The resulting color depth is positively correlated with the concentration of INS in the sample.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Mouse insulin (INS) ELISA kit (1.56-100 mIU/L) is used to quantify INS in mouse serum, plasma, cell culture supernatant, and organizations of mouse, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Mouse serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 1.56-100 mIU/L

Sensitivity: 0.6 mIU/L

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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