Mouse hormone-sensitive lipase (HSL) ELISA kit (15.625-1000 pg/mL)

Cat. No.: 0126WXX-892

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: HSL

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Mouse hormone-sensitive lipase (HSL) ELISA kit (15.625-1000 pg/mL)

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Product Overview

Description: Mouse hormone-sensitive lipase (HSL) ELISA kit (15.625-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSL in mice samples with a range of 15.625-1000 pg/mL and a minimum detectable dose (sensitivity) of 9.375 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay is based on sandwich enzyme-linked immunosorbent assay technology. An anti-HSL antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-HSL antibody serves as the detection agent. Standards, samples, and the biotinylated detection antibody are added to the wells in sequence and washed with buffer. HRP-Streptavidin is then introduced, and unbound conjugates are cleared via a wash step. TMB substrates are used to visualize the HRP enzymatic reaction, producing a blue product that shifts to yellow upon adding an acidic stop solution. The intensity of the yellow color is directly proportional to the amount of HSL captured. The absorbance is read at 450 nm, and the target concentration is calculated accordingly.

Background: HSL is the key enzyme for fat breakdown. Belonging to the “GDXG” lipase family, this enzyme primarily hydrolyzes stored triacylglycerols into free fatty acids in adipose tissue and the heart. This process represents the rate-limiting step of lipolysis, controlling the overall speed of fat breakdown. HSL can be rapidly activated by catecholamine hormones (such as epinephrine) through a cyclic adenosine monophosphate (cAMP)-dependent phosphorylation process, while insulin regulates its dephosphorylation and inactivation. Due to its functional characteristics, HSL has become a central subject in obesity research, particularly in the regulation of fat storage and release.

Synonyms: LHS; LIPE; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Mouse hormone-sensitive lipase (HSL) ELISA kit (15.625-1000 pg/mL) is used to quantify HSL in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of mouse, providing data to support research in a wide range of areas, including metabolic pathway, obesity, etc.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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