Mouse growth hormone (GH) ELISA kit (0.05-30 ng/mL)

Cat. No.: 0126WXX-120

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: GH

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Mouse growth hormone (GH) ELISA kit (0.05-30 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse growth hormone (GH) ELISA kit (0.05-30 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in mice samples with a range of 0.05-30 ng/mL and a minimum detectable dose (sensitivity) of 0.027 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a mouse GH antibody. GH in the sample binds to the immobilized antibody, followed by the addition of a biotinylated mouse GH antibody. Subsequently, streptavidin-HRP is introduced. After a washing step to remove unbound reagents, a substrate solution is added. Color develops in direct proportion to the amount of mouse GH. The reaction is terminated by an acidic stop solution, and the absorbance is recorded at 450 nm.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Mouse growth hormone (GH) ELISA kit (0.05-30 ng/mL) is used to quantify GH in serum, plasma, cell culture supernates, ascites, tissue homogenates, and other biological fluids of mouse, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Ascites; Tissue homogenates; Other biological fluids

Detection Range: 0.05-30 ng/mL

Sensitivity: 0.027 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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