Mouse glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-306

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: GSH-Px

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Mouse glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

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Product Overview

Description: Mouse glutathione peroxidase (GSH-Px) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GSH-Px in mouse samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The GSH-Px ELISA kit applies a competitive enzyme immunoassay format utilizing an anti-GSH-Px antibody and a GSH-Px-HRP conjugate. The assay sample and buffer are incubated together with the GSH-Px-HRP conjugate in the pre-coated plate for one hour. Because the number of antibody binding sites is limited, endogenous GSH-Px and the HRP-conjugate compete for these positions. After incubation, the wells are decanted and washed five times to remove unbound material. The wells are then incubated with an HRP substrate to produce a blue colored product, which shifts to yellow after the reaction is terminated with a stop solution. The resulting color intensity, measured at 450 nm, is inversely proportional to the GSH-Px concentration. The concentration of GSH-Px in each sample is determined by comparing its O.D. to the established standard curve.

Background: Glutathione peroxidase is a family of antioxidant enzymes widely distributed throughout the human body. Their core function is to protect cells from oxidative damage. Obesity itself is considered a chronic low-grade inflammatory state, often accompanied by significant oxidative stress. Glutathione peroxidase is a potential regulator of obesity.

Synonyms: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P; GPX3

Applications: Mouse glutathione peroxidase (GSH-Px) ELISA kit is used to quantify GSH-Px in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of mouse, providing data to support research in a wide range of areas, including oxidative stress, metabolism, obesity, and others.

Research Area: Oxidative stress; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 2.5-50 ng/mL

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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