Mouse pancreatic lipase (PL) ELISA kit (7.813-500 pg/mL)

Cat. No.: 0126WXX-1334

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: PL

Size:

Mouse pancreatic lipase (PL) ELISA kit (7.813-500 pg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Mouse pancreatic lipase (PL) ELISA kit (7.813-500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PL in mouse samples with a range of 7.813-500 pg/mL and a minimum detectable dose (sensitivity) of 4.688 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich-ELISA principle. The microtiter plate is pre-coated with an antibody specific to mouse PL. Samples or standards are added to the wells to combine with the immobilized antibody. Subsequently, a biotinylated detection antibody specific for mouse PL and an avidin-HRP conjugate are added successively and incubated. After washing away unbound components, a substrate solution is added. The resulting blue color turns yellow upon adding the stop solution. The O.D. value, measured at 450 ± 2 nm, is proportional to the mouse PL concentration, which can be determined via the standard curve.

Background: PL, a key member of the lipase gene family located at chromosomal position 10q26.1, is a specialized carboxyl esterase (EC 3.1.1.3) specifically expressed in the pancreas and secreted into the extracellular region. This hydrolase is essential for the efficient digestion of dietary fats, specifically driving the hydrolysis of insoluble, emulsified triglycerides into absorbable components. Beyond its primary role in lipid catabolism and cholesterol absorption, PNLIP is involved in retinoid metabolic processes and is the central molecular driver in Pancreatic Lipase Deficiency, making it a fundamental target for understanding gastrointestinal lipid digestion and its systemic impact on energy balance.

Synonyms: PNLIP; PTL; Lipase; pancreatic; Pancreatic triacylglycerol lipase

Applications: Mouse pancreatic lipase (PL) ELISA kit (7.813-500 pg/mL) is used to quantify PL in serum, plasma, and biological fluids of mice, providing data to support research in a wide range of areas, including enzyme and kinase, metabolic pathway, Infection immunity, hepatology, obesity, and others.

Research Area: Enzyme & Kinase; Metabolic pathway; Infection immunity; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 7.813-500 pg/mL

Sensitivity: 4.688 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 86-112%

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out