Monkey peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-10 ng/mL)

Cat. No.: 0126WXX-1485

Assay Type: Quantitative sandwich ELISA

Target Species: Monkey

Assay Target: PPARα

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Monkey peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.5-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Monkey peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in monkey samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PPARα ELISA kit utilizes a competitive enzyme immunoassay technique with a monoclonal anti-PPARα antibody and a PPARα-HRP conjugate. Sample PPARα and the conjugate compete for binding sites on the pre-coated plate during a one-hour incubation. Following the five wash steps, an HRP substrate is added to produce a colored complex. The reaction is halted with a stop solution, and the intensity is measured at 450 nm. The color intensity is inversely proportional to the concentration of PPARα in the sample.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Monkey peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative sandwich is used to quantify PPARα in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of monkey, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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