Human adipose triglyceride lipase (ATGL) ELISA kit (6.25-400 mIU/mL)

Cat. No.: 0126WXX-1406

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ATGL

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Human adipose triglyceride lipase (ATGL) ELISA kit (6.25-400 mIU/mL)

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Product Overview

Description: Human adipose triglyceride lipase (ATGL) ELISA kit (6.25-400 mIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ATGL in human samples with a range of 6.25-400 mIU/mL and a minimum detectable dose (sensitivity) of <1.56 mIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a quantitative sandwich enzyme immunoassay technique. An antibody specific for ATGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, allowing any ATGL present to be captured by the immobilized antibody. After the removal of unbound substances through a washing step, a biotin-conjugated detection antibody specific for ATGL is introduced to the wells. Following an additional wash, avidin-conjugated HRP is added. After a final wash to remove any remaining unbound reagents, a substrate solution is added to the wells, and color develops in direct proportion to the amount of ATGL bound in the initial step. The color development is stopped, and the intensity of the signal is measured to calculate the final concentration.

Background: ATGL, also known as PNPLA2, is the rate-limiting enzyme in the intracellular lipolysis pathway and a key member of the Triglyceride Lipase (TGL) family. ATGL primarily catalyzes the first step in the hydrolysis of triglycerides (TG) stored in cellular lipid droplets into diglycerides and free fatty acids (FFA). In obesity research, ATGL occupies a central position as it directly governs energy storage and mobilization. Dysregulation of ATGL activity leads to ectopic TG accumulation in non-adipose tissues such as the liver and muscle, triggering lipotoxicity and associated pathological responses. Furthermore, ATGL activity influences FFA release, thereby affecting systemic energy balance and adipose tissue inflammation. Consequently, ATGLs represent key targets for understanding lipolysis dysregulation and obesity-related metabolic complications.

Synonyms: PNPLA2; PEDF-R; TTS2; Pigment epithelium-derived factor; Transport-secretion protein 2; Patatin like phospholipase domain containing protein 2; Calcium-independent phospholipase A2; Desnutrin

Applications: Human adipose triglyceride lipase (ATGL) ELISA kit (6.25-400 mIU/mL) is used to quantify ATGL in serum, urine, and tissue homogenates of humans, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Serum; Urine; Tissue homogenates

Detection Range: 6.25-400 mIU/mL

Sensitivity: <1.56 mIU/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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