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Human prothrombin fragment 1 + 2 (F1 + 2) ELISA kit (1.56-100 ng/mL)
Cat. No.:
OB0625WXX-763
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
F1 + 2
Size:
Add to basket
Product Overview
Description:
Human prothrombin fragment 1 + 2 (F1 + 2) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of F1 + 2 in human with a range of 1.56-100 ng/mL and a specificity of up to 0.5 ng/mL.
Assay Principle:
The pre-coated antibody is a human F1+2 monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. The samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells. The reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue under the catalysis of peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
Background:
Thrombin converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Research indicates that obesity is associated with disturbances in coagulation balance, leading to a pro-coagulant and hypofibrinolytic state. Analysis of F1 + 2 levels is of certain significance.
Synonyms:
PF1+2; Prothrombin fragment 1.2; Prothrombin fragment F1+2
Applications:
Human prothrombin fragment 1 + 2 (F1 + 2) ELISA kit (1.56-100 ng/mL) is used to quantify F1 + 2 in human serum, plasma, and cell culture supernatant samples of human, providing data to support a wide range of studies.
Research Area:
Blood coagulation regulation; Obesity research
Specification
Sample Type:
Human serum; Plasma; Cell culture supernatant
Detection Range:
1.56-100 ng/mL
Sensitivity:
Up to 0.5 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
