Human proinsulin (PI) ELISA kit (31.25-2000 pg/mL)

Cat. No.: 0126WXX-1275

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PI

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Human proinsulin (PI) ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Human proinsulin (PI) ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PI in human samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of 18.75 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate has been pre-immobilized with an antibody specific to PI. Upon adding standards or samples, a biotin-conjugated detection antibody for PI is introduced into the wells. Following this, avidin-conjugated HRP is added and incubated. After the TMB substrate is introduced, only wells containing the PI "sandwich" complex will exhibit a colorimetric change. The enzymatic reaction is terminated with sulphuric acid, and the O.D. is recorded at 450 nm ± 10 nm. The final PI concentration is interpolated from the generated standard curve.

Background: Proinsulin is the precursor molecule of insulin, synthesized in pancreatic β cells and subsequently processed into mature insulin and C-peptide. It plays a critical role in glucose homeostasis by serving as an essential intermediate in insulin biosynthesis and secretion. Under normal physiological conditions, proinsulin is efficiently converted to insulin; however, impaired processing or increased proinsulin secretion reflects β-cell dysfunction. Elevated circulating proinsulin levels are commonly observed in obesity and insulin resistance, making proinsulin a key indicator of metabolic stress and a contributing factor to the development of obesity-associated type 2 diabetes.

Synonyms: INS; Pro-Insulin; Preproinsulin

Applications: Human proinsulin (PI) ELISA kit (31.25-2000 pg/mL) is used to quantify PI in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including endocrinology, hormone metabolism, obesity, and others.

Research Area: Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 31.25-2000 pg/mL

Sensitivity: 18.75 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 85-105%

Storage: Store at -20°C (microplate; standard) and 2-8°C (other reagents).

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