Human NADP-dependent malic enzyme ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1028

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: NADP-dependent malic enzyme

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Human NADP-dependent malic enzyme ELISA kit-Quantitative sandwich

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Product Overview

Description: Human NADP-dependent malic enzyme ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NADP-dependent malic enzyme in human samples with a range of 0.2- 60 ng/mL and a minimum detectable dose (sensitivity) of 0.107 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on NADP-dependent malic enzyme antibody-NADP-dependent malic enzyme antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NADP-dependent malic enzyme antigen targets in samples.

Background: NADP-dependent malic enzyme is a crucial metabolic enzyme. Its primary function is to catalyze the conversion of malate to pyruvate while generating a key cofactor—reduced nicotinamide adenine dinucleotide phosphate (NADPH). This renders it essential for fatty acid and cholesterol synthesis, as well as antioxidant defense mechanisms. Obesity constitutes a chronic inflammatory state frequently accompanied by elevated oxidative stress levels within cells and tissues. Investigating alterations in NADP-dependent malic enzyme-related pathways offers deeper insights into the pathophysiology of obesity.

Synonyms: Malic enzyme; ME; ME1; NADPH-malic enzyme; Decarboxylating malate dehydrogenase; Malate dehydrogenase (decarboxylating) (NADP⁺)

Applications: Human NADP-dependent malic enzyme ELISA kit-Quantitative sandwich is used to quantify NADP-dependent malic enzyme in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including metabolism, oxidative stress, obesity, etc.

Research Area: Metabolism; Oxidative stress; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.2- 60 ng/mL

Sensitivity: 0.107 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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