Human Meprin A subunit alpha (MEP1A) ELISA kit (0.5-100 ng/mL)

Cat. No.: 0126WXX-1040

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: MEP1A

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Human Meprin A subunit alpha (MEP1A) ELISA kit (0.5-100 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human Meprin A subunit alpha (MEP1A) ELISA kit (0.5-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of MEP1A in human samples with a range of 0.5-100 ng/mL and a minimum detectable dose (sensitivity) of 0.27 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is an ELISA based on the sandwich principle. The plate is pre-coated with a human MEP1A antibody. MEP1A present in the sample binds to the antibodies immobilized on the wells, followed by the addition of a biotinylated human MEP1A detection antibody. Subsequently, streptavidin-HRP is introduced to bind the biotinylated antibody. After incubation, unbound streptavidin-HRP is removed during a washing step. Substrate solution is then added, and color develops in direct proportion to the amount of human MEP1A present. The reaction is terminated by an acidic stop solution, and the absorbance is recorded at 450 nm.

Background: MEP1A is a constituent subunit of the zinc-dependent metalloproteinase Meprin A, highly expressed primarily in the renal brush border and intestine. Its core function is as an endopeptidase that hydrolyzes peptide bonds, participating in protein digestion, processing specific peptides, and degrading the extracellular matrix (ECM). It is associated with glucose and insulin metabolism.

Synonyms: Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA

Applications: Human Meprin A subunit alpha (MEP1A) ELISA kit (0.5-100 ng/mL) is used to quantify MEP1A in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including metabolism, etc.

Research Area: Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.5-100 ng/mL

Sensitivity: 0.27 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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