Human lipoprotein lipase (LPL) ELISA kit (50-1600 U/L)

Cat. No.: 0126WXX-948

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LPL

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Human lipoprotein lipase (LPL) ELISA kit (50-1600 U/L)

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Product Overview

Description: Human lipoprotein lipase (LPL) ELISA kit (50-1600 U/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in human samples with a range of 50-1600 U/L and a minimum detectable dose (sensitivity) of 10 U/L. The kit is highly sensitive and easy to use.

Assay Principle: Purified human LPL antibody is used to coat microelisa strip plate wells to create a solid-phase antibody. LPL and HRP-labeled LPL antibody are added to form an antibody-antigen-antibody-enzyme complex. After complete washing, the TMB substrate solution is added, turning blue under HRP catalysis. The reaction is terminated with a sulphuric acid solution, and the color change is measured spectrophotometrically at 450 nm. The LPL concentration is then determined by comparing the sample O.D. to the standard curve.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Human lipoprotein lipase (LPL) ELISA kit (50-1600 U/L) is used to quantify LPL in serum, plasma, tissue homogenates, feces, urine, and body fluids of human, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenate; Feces; Urine; Body fluids

Detection Range: 50-1600 U/L

Sensitivity: 10 U/L

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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