Porcine C-reactive protein (CRP) ELISA kit (1.563-100 pg/mL)

Cat. No.: OB0625WXX-457

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: CRP

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Porcine C-reactive protein (CRP) ELISA kit (1.563-100 pg/mL)

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Product Overview

Description: Porcine C-reactive protein (CRP) ELISA kit (1.563-100 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in porcine with a range of 1.563-100 pg/mL and a specificity of 0.938 pg/mL.

Assay Principle: The anti-CRP antibody is pre-coated onto a 96-well plate. The biotin-conjugated anti-CRP antibody is used as the detection antibody. Standards, assay samples, and biotin-conjugated detection antibody are then added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzymatic reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of CRP sample captured in the plate. The concentration of CRP can be calculated by reading the absorbance at 450nm with a microplate reader.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Porcine C-reactive protein (CRP) ELISA kit (1.563-100 pg/mL) is used to quantify CRP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of porcine, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 1.563-100 pg/mL

Sensitivity: 0.938 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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