Human lipopolysaccharide binding protein (LBP) ELISA kit (31.25-2000 pg/mL)

Cat. No.: 0126WXX-764

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LBP

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Human lipopolysaccharide binding protein (LBP) ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Human lipopolysaccharide binding protein (LBP) ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in human samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of 18.75 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: A capture antibody is pre-coated onto 96-well plates, and a biotin-conjugated antibody is used for detection. Standards, samples, and the detection antibody are added sequentially and washed. HRP-streptavidin is then introduced, and unbound conjugates are removed with the wash buffer. TMB substrate is used to visualize the HRP enzymatic reaction, producing a blue color that turns yellow upon adding acidic stop solution. The yellow signal density is proportional to the amount of target captured, measured at 450 nm.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Human lipopolysaccharide binding protein (LBP) ELISA kit (31.25-2000 pg/mL) is used to quantify LBP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 31.25-2000 pg/mL

Sensitivity: 18.75 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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