Human insulin (INS) ELISA kit (0-140 μIU/mL)

Cat. No.: 0126WXX-586

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: INS

Size:

Human insulin (INS) ELISA kit (0-140 μIU/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human insulin (INS) ELISA kit (0-140 μIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 0-140 μIU/mL and a minimum detectable dose (sensitivity) of <5 μIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay is based on sandwich ELISA technology, featuring 96-well plates pre-coated with an anti-insulin monoclonal antibody. Standards, samples, and an HRP-conjugated anti-insulin polyclonal detection antibody are added sequentially. Following a wash cycle, TMB substrate is added to visualize the HRP enzymatic reaction, yielding a blue product that shifts to yellow upon acidification. The density of the yellow color is proportional to the insulin concentration, which is measured at 450 nm. Background: Insulin, synthesized by pancreatic beta cells, consists of A and B chains linked by disulfide bonds and plays a critical role in glucose transport and metabolic anti-inflammatory effects.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (0-140 μIU/mL) is used to quantify INS in serum, plasma, body fluids, tissue lysates, and cell culture supernatants of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Body Fluids; Tissue lysates; Cell culture supernatants

Detection Range: 0-140 μIU/mL

Sensitivity: <5 μIU/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out