Human insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.313-20 ng/mL)

Cat. No.: 0126WXX-477

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: IGFBP-2

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Human insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.313-20 ng/mL)

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Product Overview

Description: Human insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.313-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IGFBP-2 in human samples with a range of 0.313-20 ng/mL and a minimum detectable dose (sensitivity) of 0.188 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-human IGFBP-2 antibody is pre-coated onto 96-well plates. A biotin-conjugated detection antibody and test samples are added sequentially. After washing with the wash buffer, HRP-streptavidin is introduced. TMB substrates are used to visualize the HRP enzymatic reaction, producing a blue product that turns yellow upon the addition of an acidic stop solution. The yellow intensity is proportional to the human IGFBP-2 concentration, which is calculated by reading the O.D. at 450 nm.

Background: IGFBP-2 is a multifunctional molecule that plays a crucial role in human growth, metabolism, and cellular processes by regulating the biological activity of insulin-like growth factors (IGFs). Primarily through binding to IGF proteins, IGFBP-2 prolongs their half-life in the body and modulates their interactions with cell surface receptors, thereby inhibiting or enhancing the pro-growth effects of IGFs. The role of IGFBP-2 in obesity has garnered increasing attention. Its reduced levels are closely associated with obesity, dyslipidemia, and insulin resistance.

Synonyms: IBP-2; IGF-binding protein 2; IGFBP2; BP2, IBP2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; Insulin-Like Growth Factor-Binding Protein 2

Applications: Human insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.313-20 ng/mL) is used to quantify IGFBP-2 in serum, plasma, cell culture supernatant, cell, tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including immunity, endocrinology, signal transduction, obesity, and others.

Research Area: Immunity; Endocrinology; Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell; Tissue lysate; Other liquid samples

Detection Range: 0.313-20 ng/mL

Sensitivity: 0.188 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-99%

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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