Mouse peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL)

Cat. No.: 0126WXX-1506

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: PPARδ

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Mouse peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL)

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Product Overview

Description: Mouse peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARδ in mouse samples with a range of 31.2-2000 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to the target antigen. Standards or samples are introduced into the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for the target antigen, followed by the addition of Avidin-conjugated HRP and incubation. A TMB substrate solution is then added to each well. Only wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the target antigen in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: Peroxisome proliferator-activated receptor delta (PPARδ) is a ligand-activated nuclear receptor that regulates gene expression involved in fatty acid transport, beta-oxidation, and lipid metabolism. Activated by polyunsaturated fatty acids and other ligands, PPARδ promotes energy expenditure, fatty acid catabolism, and transcription of metabolic genes, contributing to the regulation of glucose and lipid homeostasis. Dysregulation of PPARδ activity can impair lipid metabolism and energy balance, linking it to obesity and related metabolic disorders.

Synonyms: PPARD; PPARd; PPAR-delta; Nuclear hormone receptor 1; NUC1

Applications: Mouse peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL) is used to quantify PPARδ in serum, plasma, tissue homogenates, cell culture supernatant, and other biological fluids of mice, providing data to support research in a wide range of areas, including signal transduction, metabolic pathways, obesity, and others.

Research Area: Signal transduction; Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernatants; Other biological fluids

Detection Range: 31.2-2000 pg/mL

Precision (Intra-assay): CV≤4.6%

Precision (Inter-assay): CV≤7.5%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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