Human insulin (INS) ELISA kit (1.76-100 μIU/mL)

Cat. No.: 0126WXX-577

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: INS

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Human insulin (INS) ELISA kit (1.76-100 μIU/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human insulin (INS) ELISA kit (1.76-100 μIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 1.76-100 μIU/mL and a minimum detectable dose (sensitivity) of 1.76 μIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This Insulin ELISA kit is a solid-phase assay based on the sandwich principle. Microtiter wells are coated with a monoclonal antibody specific to a unique antigenic site on the insulin molecule. Endogenous insulin from the sample is incubated with a biotin-conjugated anti-insulin antibody. During a second incubation step, a streptavidin-peroxidase enzyme complex binds to the biotinylated antibody. The amount of bound HRP complex is directly proportional to the insulin concentration, which is measured by the intensity of the color developed after substrate addition.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (1.76-100 μIU/mL) is used to quantify INS in serum and plasma of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma

Detection Range: 1.76-100 μIU/mL

Sensitivity: 1.76 μIU/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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