Human hormone-sensitive lipase (HSL) ELISA kit (1.56-100 U/L)

Cat. No.: 0126WXX-887

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: HSL

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Human hormone-sensitive lipase (HSL) ELISA kit (1.56-100 U/L)

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Product Overview

Description: Human hormone-sensitive lipase (HSL) ELISA kit (1.56-100 U/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSL in human samples with a range of 1.56-100 U/L and a minimum detectable dose (sensitivity) of 0.89 U/L. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on HSL antibody-HSL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSL antigen targets in samples.

Background: HSL is the key enzyme for fat breakdown. Belonging to the “GDXG” lipase family, this enzyme primarily hydrolyzes stored triacylglycerols into free fatty acids in adipose tissue and the heart. This process represents the rate-limiting step of lipolysis, controlling the overall speed of fat breakdown. HSL can be rapidly activated by catecholamine hormones (such as epinephrine) through a cyclic adenosine monophosphate (cAMP)-dependent phosphorylation process, while insulin regulates its dephosphorylation and inactivation. Due to its functional characteristics, HSL has become a central subject in obesity research, particularly in the regulation of fat storage and release.

Synonyms: LHS; LIPE; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Human hormone-sensitive lipase (HSL) ELISA kit (1.56-100 U/L) is used to quantify HSL in serum, plasma, whole blood, tissue, saliva, urine, feces, and other fluids of human, providing data to support research in a wide range of areas, including metabolic pathway, obesity, etc.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Whole blood; Other fluid; Tissue; Saliva; Urine; Feces

Detection Range: 1.56-100 U/L

Sensitivity: 0.89 U/L

Precision (Intra-assay): CV≤7.1%

Precision (Inter-assay): CV≤10.9%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 0.95

Storage: Store at -20°C.

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