Human necdin ELISA kit (0.313-20 ng/mL, 0.188 ng/mL)

Cat. No.: 0126WXX-1062

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: Necdin

Size:

Human necdin ELISA kit (0.313-20 ng/mL, 0.188 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human necdin ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of necdin in human samples with a range of 0.313-20 ng/mL and a minimum detectable dose (sensitivity) of 0.188 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-human necdin antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-human necdin antibody is utilized as the detection antibody. Standards, test samples, and the biotinylated detection antibody are added to the wells sequentially, followed by a wash step with wash buffer. HRP-streptavidin is then introduced, and unbound conjugates are removed through further washing. TMB substrates are used to visualize the HRP enzymatic reaction; the TMB is catalyzed by HRP to produce a blue product that shifts to yellow upon the addition of an acidic stop solution. The intensity of the yellow color is directly proportional to the amount of human necdin captured in the plate. The O.D. absorbance is measured at 450 nm in a microplate reader, allowing for the calculation of the human necdin concentration.

Background: Necdin is a protein located within neuronal cell nuclei and is considered a transcriptional repressor. It belongs to the genes within the critical region of Prader-Willi syndrome (PWS). PWS is a genetic disorder characterized by severe morbid obesity and hyperphagia, primarily attributed to the deletion or dysfunction of genes within the PWS region (including Necdin). This suggests Necdin participates in the normal suppression of satiety and food intake within the hypothalamus.

Synonyms: NDN

Applications: Human necdin ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is used to quantify necdin in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including appetite regulation, signal transduction, obesity, etc.

Research Area: Appetite regulation; Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.313-20 ng/mL

Sensitivity: 0.188 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out