Human glucagon-like peptide 1 receptor (GLP-1R) ELISA kit (0.05-30 ng/mL)

Cat. No.: 0126WXX-198

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: GLP-1R

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Human glucagon-like peptide 1 receptor (GLP-1R) ELISA kit (0.05-30 ng/mL)

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Product Overview

Description: Human glucagon-like peptide 1 receptor (GLP-1R) ELISA kit (0.05-30 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GLP-1R in human samples with a range of 0.05-30 ng/mL and a minimum detectable dose (sensitivity) of 0.026 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a human GLP-1R antibody. When samples are added, GLP-1R binds to the immobilized capture antibodies. Subsequently, a biotinylated human GLP-1R antibody is introduced to bind the captured analyte. Streptavidin-HRP is then added to bind the biotinylated detection antibody. After incubation and a washing step to remove unbound reagents, a substrate solution is added; color develops in direct proportion to the concentration of human GLP-1R. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: GLP-1R is a G protein-coupled receptor located on the cell membrane. It serves as the primary receptor for the endogenous hormone GLP-1 and is widely distributed across multiple tissues, including pancreatic β-cells, the hypothalamus, the gastrointestinal tract, and the heart. When GLP-1 binds to and activates the GLP-1R, it initiates a series of intracellular signaling pathways that influence insulin secretion, blood glucose homeostasis, appetite, and gastrointestinal motility. The GLP-1R plays a pivotal role in obesity treatment and is currently one of the most effective and sought-after targets for anti-obesity drugs. Activating this receptor transmits potent satiety signals to the brain, effectively suppressing appetite and reducing cravings for high-calorie foods.

Synonyms: GLP 1 receptor; GLP-1 receptor; GLP1R

Applications: Human glucagon-like peptide 1 receptor (GLP-1R) ELISA kit (0.05-30 ng/mL) is used to quantify GLP-1R in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including endocrinology, cardiovascular, obesity, and others.

Research Area: Endocrinology; Cardiovascular; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.05-30 ng/mL

Sensitivity: 0.026 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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