Guinea pig glucagon (GCG) ELISA kit (31.2-2000 pg/mL)

Cat. No.: 0126WXX-59

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: GCG

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Guinea pig glucagon (GCG) ELISA kit (31.2-2000 pg/mL)

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Product Overview

Description: Guinea pig glucagon (GCG) ELISA kit (31.2-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in guinea pig samples with a range of 31.2-2000 pg/mL and a minimum detectable dose (sensitivity) of 12 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit includes a microplate pre-coated with a guinea pig GCG monoclonal capture antibody. After samples are added, the GCG antigen is captured by the immobilized antibody. The wells are then washed with PBS or TBS. A biotin-labeled polyclonal detection antibody is introduced to form a sandwich complex. Following another wash, an avidin-peroxidase conjugate is added to bind to the biotinylated antibody. A final wash removes unbound reagents before the TMB substrate is added. The HRP enzyme catalyzes a color change to blue, which turns yellow upon adding a stop solution. The final intensity is directly proportional to the GCG concentration.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Guinea pig glucagon (GCG) ELISA kit (31.2-2000 pg/mL) is used to quantify GCG in serum, and plasma, cell culture supernatant, and organizations of guinea pig, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 31.2-2000 pg/mL

Sensitivity: 12 pg/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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