Sheep peptide YY (PYY) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1679

Assay Type: Quantitative competitive ELISA

Target Species: Sheep

Assay Target: PYY

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Sheep peptide YY (PYY) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Sheep peptide YY (PYY) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in sheep samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PYY antibody and a PYY-HRP conjugate. The assay sample and buffer are incubated alongside the conjugate in the pre-coated microplate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then treated with an HRP enzyme substrate to produce a blue colored complex, which shifts to yellow after the reaction is terminated with a stop solution. The intensity of the color is measured spectrophotometrically at 450 nm. Because PYY from the samples competes with the PYY-HRP conjugate for a limited number of antibody binding sites, the color intensity is inversely proportional to the PYY concentration in the sample.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Sheep peptide YY (PYY) ELISA kit-Quantitative competitive is used to quantify PYY in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of sheep, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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