Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1498

Assay Type: Quantitative competitive ELISA

Target Species: Porcine

Assay Target: PPARα

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Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive

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Product Overview

Description: Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in porcine samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PPARα ELISA kit applies a competitive enzyme immunoassay technique utilizing a monoclonal anti-PPARα antibody and a PPARα-HRP conjugate. Samples and buffers are incubated with the PPARα-HRP conjugate in the pre-coated plate for one hour, allowing them to compete for a limited number of antibody binding sites. After incubation, the wells are decanted and washed five times to remove unbound materials. A substrate for the HRP enzyme is then added, producing a blue complex that turns yellow upon the addition of a stop solution. The intensity of the color, measured at 450 nm, is inversely proportional to the PPARα concentration in the sample. As more binding sites are occupied by sample-derived PPARα, fewer sites remain available for the PPARα-HRP conjugate. A standard curve is used to interpolate the exact concentration of PPARα in each sample.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive is used to quantify PPARα in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of porcine, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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