Human glucagon (GCG) ELISA kit (7 pg/mL)

Cat. No.: 0126WXX-88

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: GCG

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Human glucagon (GCG) ELISA kit (7 pg/mL)

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Product Overview

Description: Human glucagon (GCG) ELISA kit (7 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in human samples with a minimum detectable dose (sensitivity) of 7 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a sandwich ELISA technique to quantify human GCG. A specific capture antibody has been pre-coated onto a 96-well microplate. After adding standards and samples, any GCG present is bound by the immobilized antibody. Following a wash, a biotinylated detection antibody is introduced. After another wash, HRP-conjugated streptavidin is added. A final wash removes unbound conjugate before the TMB substrate is introduced. Color develops in direct proportion to the amount of GCG bound. The reaction is stopped with a stop solution, shifting the color from blue to yellow, and the intensity is measured at 450 nm.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Human glucagon (GCG) ELISA kit (7 pg/mL) is used to quantify GCG in cell culture supernatants, serum, and plasma of human, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Cell culture supernatants; Serum; Plasma

Sensitivity: 7 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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