Mouse pro-opiomelanocortin (POMC) ELISA kit (47-3000 pg/mL)

Cat. No.: 0126WXX-1441

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: POMC

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Mouse pro-opiomelanocortin (POMC) ELISA kit (47-3000 pg/mL)

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Product Overview

Description: Mouse pro-opiomelanocortin (POMC) ELISA kit (47-3000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in mouse samples with a range of 47-3000 pg/mL and a minimum detectable dose (sensitivity) of <23.5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a competitive inhibition enzyme immunoassay technique. The microtiter plate is pre-coated with the POMC antigen. Standards or samples are added to the wells with an antibody specific for POMC and an HRP-conjugated goat-anti-rabbit antibody. A competitive reaction occurs between the pre-coated POMC and the POMC in the samples for the specific antibody. Upon addition of the substrate solution, color develops in inverse proportion to the amount of POMC in the sample. The reaction is stopped, and the intensity of the color is measured.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Mouse pro-opiomelanocortin (POMC) ELISA kit (47-3000 pg/mL) is used to quantify POMC in serum, plasma, cell culture supernatant, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Tissue homogenates

Detection Range: 47-3000 pg/mL

Sensitivity: <23.5 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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