Goat insulin (INS) ELISA kit (1.56-100 mIU/L)

Cat. No.: 0126WXX-557

Assay Type: Quantitative sandwich ELISA

Target Species: Goat

Assay Target: INS

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Goat insulin (INS) ELISA kit (1.56-100 mIU/L)

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Product Overview

Description: Goat insulin (INS) ELISA kit (1.56-100 mIU/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in goat samples with a range of 1.56-100 mIU/L and a minimum detectable dose (sensitivity) of 0.5 mIU/L. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a double-antibody sandwich ELISA technique. The microplate is pre-coated with a goat INS monoclonal antibody, used in conjunction with a biotin-labeled polyclonal detection antibody. Samples and detection antibodies are incubated in the wells and washed with PBS or TBS, followed by the addition of Avidin-peroxidase conjugates. A TMB substrate is used for color development, producing a blue signal that turns yellow under acidic conditions. The depth of the color is positively correlated with the concentration of INS in the sample.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Goat insulin (INS) ELISA kit (1.56-100 mIU/L) is used to quantify INS in goat serum, plasma, cell culture supernatant, and organizations of goat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Goat serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 1.56-100 mIU/L

Sensitivity: 0.5 mIU/L

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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