Human cathepsin S (CTSS) ELISA kit (1.25-80 ng/mL)

Cat. No.: OB0625WXX-526

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: CTSS

Size:

Human cathepsin S (CTSS) ELISA kit (1.25-80 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human cathepsin S (CTSS) ELISA kit (1.25-80 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSS in human with a range of 1.25-80 ng/mL and a specificity of 0.31 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for CTSS. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for CTSS. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only wells containing CTSS, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of CTSS in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: CTSS is an important member of the cysteine protease family. As a potent mammalian elastase, it can degrade various extracellular components, including fibronectin, laminin, elastin, and collagen. CTSS is abnormally expressed in various disease states, such as obesity and atherosclerosis, which makes it a biomarker and a potential therapeutic target. Studies have found that CTSS inhibitors may be a possible strategy for treating obesity.

Synonyms: CTS-S; EC 3.4.22.27

EC NO.: 3.4.22.27

Applications: Human cathepsin S (CTSS) ELISA kit (1.25-80 ng/mL) is used to quantify CTSS in serum, plasma, cell culture supernates, tissue homogenates, cell lysates samples of human, providing data to support a wide range of studies.

Research Area: Obesity research; Immune response; Signaling pathway; Protein degradation

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Tissue homogenates; Cell lysates

Detection Range: 1.25-80 ng/mL

Sensitivity: 0.31 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out