Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.094 ng/mL)

Cat. No.: OB0625WXX-349

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: CB1

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Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.094 ng/mL)

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Product Overview

Description: Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.094 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CB1 in human with a range of 0.156-10 ng/mL and a specificity of 0.094 ng/mL.

Assay Principle: The anti-human CB1 antibody is pre-coated onto a 96-well plate. Biotin-conjugated anti-human CB1 antibody is used as the detection antibody. Subsequently, standards, test samples, and biotin-conjugated detection antibody are added to the wells and washed with buffer. HRP-streptavidin is added, and unbound conjugate is washed away with washing solution. TMB substrate is used to observe the HRP enzymatic reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of yellow color is proportional to the amount of human CB1 sample captured in the plate. The concentration of human CB1 can be calculated by reading the absorbance at 450nm with a microplate reader.

Background: CB1 is a member of the cannabinoid receptor family and the G protein-coupled receptor (GPCR) superfamily. It is expressed at the highest levels in different brain regions, and is also present at lower levels in most other cell/tissue types, including adipose tissue. It is thought to activate multiple signaling pathways, regulating energy metabolism and cell survival/death. CB1 has become a drug target for treating obesity. CB1 inhibitors may be potential anti-obesity drugs.

Synonyms: CNR1; Central cannabinoid receptor; Brain-type cannabinoid receptor

Applications: Human brain cannabinoid receptor 1 (CB1) ELISA kit (0.156-10 ng/mL, 0.094 ng/mL) is used to quantify CB1 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support a wide range of studies.

Research Area: Lipolysis; Glucose uptake; Energy metabolism; Cell growth; Signaling pathway; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.094 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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