Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.12 ng/mL)

Cat. No.: OB0525WXX-215

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ADP

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Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.12 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.12 ng/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 0.156-10 ng/mL and a sensitivity of 0.12 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with a specific ADP antibody. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated ADP-specific monoclonal antibody preparation, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.12 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in human biological samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Biological samples

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.12 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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