Bovine leptin receptor (LEPR) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1209

Assay Type: Quantitative competitive ELISA

Target Species: Bovine

Assay Target: LEPR

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Bovine leptin receptor (LEPR) ELISA kit-Quantitative competitive

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Product Overview

Description: Bovine leptin receptor (LEPR) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in porcine samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This method utilizes a monoclonal anti-LEPR antibody and an LEPR-HRP conjugate. Samples and assay buffers are co-incubated with the HRP-conjugate in a pre-coated microplate for sixty minutes. In this system, endogenous LEPR and the enzyme-labeled conjugate compete for a finite number of antibody binding sites. After incubation, the plate is decanted and washed five times to remove unbound material. A substrate for the HRP enzyme is then added, producing a blue complex that shifts to yellow upon the addition of a stop solution. The resulting signal intensity, measured at 450 nm, is inversely proportional to the LEPR concentration. Quantitative results are interpolated from a standard curve relating O.D. to standard concentrations.

Background: LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.

Synonyms: CD295; LEPR; OBR; LR

Applications: Bovine leptin receptor (LEPR) ELISA kit-Quantitative competitive is used to quantify LEPR in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of bovine, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.

Research Area: Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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