Guinea pig cholecystokinin A receptor (CCKAR) ELISA kit

Cat. No.: OB0625WXX-292

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: CCKAR

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Guinea pig cholecystokinin A receptor (CCKAR) ELISA kit

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Product Overview

Description: Guinea pig cholecystokinin A receptor (CCKAR) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CCKAR in guinea pig. The kit is highly specific, sensitive, and easy to use.

Assay Principle: Antibodies specific for CCKAR have been pre-coated onto microtiter plates. Standards and samples are pipetted into the wells, and any CCKAR present is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for CCKAR is added to the wells. After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of CCKAR bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Background: Cholecystokinin A receptor is a G protein-coupled receptor that binds to non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. It plays a role in regulating satiety and the release of dopamine, among others.

Synonyms: CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin type 1 receptor

Applications: Guinea pig cholecystokinin A receptor (CCKAR) ELISA kit is an important tool for the quantitative detection of CCKAR in various biological samples (serum, plasma, and other biological fluids) of guinea pigs.

Research Area: Feeding behavior; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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