Goat uncoupling protein-1 (UCP1) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-2037

Assay Type: Quantitative sandwich ELISA

Target Species: Goat

Assay Target: UCP1

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Goat uncoupling protein-1 (UCP1) ELISA kit-Quantitative sandwich

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Product Overview

Description: Goat uncoupling protein-1 (UCP1) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP1 in goat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a standard double-antibody sandwich ELISA technique. The microplate is pre-coated with a goat UCP1 monoclonal antibody, and the detection agent is a biotin-labeled polyclonal antibody. Samples and biotinylated antibodies are added to the ELISA plate wells and subsequently washed with PBS or TBS. Then, avidin-peroxidase conjugates are added to the wells in sequence. After the reactants are thoroughly washed out with PBS or TBS, the TMB substrate is used for coloration. Under peroxidase catalysis, the TMB turns blue and finally shifts to yellow upon the addition of acid. The color depth is positively correlated with the concentration of target factors in the samples.

Background: Uncoupling protein 1 (UCP1) is a mitochondrial transporter that mediates proton leakage across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis and dissipating energy as heat. UCP1 plays a key role in brown adipose tissue thermogenesis and energy expenditure, contributing to the regulation of whole-body metabolism. Impaired UCP1 function can reduce thermogenic capacity and promote energy storage, linking it to obesity and related metabolic disorders.

Synonyms: SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; Uncoupling Protein 1; Mitochondrial; Mitochondrial brown fat uncoupling protein 1

Applications: Goat uncoupling protein-1 (UCP1) ELISA kit-Quantitative sandwich is used to quantify UCP1 in goat serum, plasma, and cell culture supernatant of goat, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity