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Chicken apolipoprotein C-II (APOC2) ELISA kit
Cat. No.:
OB0625WXX-121
Assay Type:
Quantitative sandwich ELISA
Target Species:
Chicken
Assay Target:
APOC2
Size:
Add to basket
Product Overview
Description:
Chicken apolipoprotein C-II (APOC2) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of APOC2 in chicken with a range of 31.25-2000 ng/mL and a specificity of 18.75 pg/mL.
Assay Principle:
The micro ELISA plates provided in this kit are pre-coated with an antibody specific for APOC2. Standards or samples are added to the appropriate micro ELISA plate wells and bound to the specific antibody. A biotinylated detection antibody specific for APOC2 and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing APOC2, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of APOC2. The concentration of APOC2 in the sample is calculated by comparing the OD value of the sample with the standard curve.
Background:
Apolipoprotein C-II is a component of very low-density lipoproteins and chylomicrons. It activates lipoprotein lipase and plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
Synonyms:
Apolipoprotein C2; APC2; APO-CII; APOC-II; Apo-C2; ProapoC-II; Proapolipoprotein C-II
Applications:
Chicken apolipoprotein C-II (APOC2) ELISA kit is used to quantify APOC2 in serum, plasma, and biological fluid samples of chicken, providing data to support a wide range of studies.
Research Area:
Metabolic pathway; Obesity research
Specification
Sample Type:
Serum; Plasma; Biological fluids
Detection Range:
31.25-2000 ng/mL
Sensitivity:
18.75 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 4°C.
