Bovine beta-endorphin (BEP) ELISA kit

Cat. No.: OB0625WXX-238

Assay Type: Quantitative competitive ELISA

Target Species: Bovine

Assay Target: BEP

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INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine beta-endorphin (BEP) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of BEP in bovine with a range of 15.625-1000 pg/mL and a specificity of 4.89 pg/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for BEP. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated BEP-specific antibody preparation. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only wells containing BEP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of BEP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: BEP is an opioid peptide derived from POMC, produced in the pituitary and hypothalamus, which acts as a neurotransmitter to modulate pain, mood, and other functions.

Synonyms: Beta EP; b-EP; bEP

Applications: Bovine beta-endorphin (BEP) ELISA kit is used to quantify BEP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of bovine, providing data to support a wide range of studies.

Research Area: Signal transduction

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 15.625-1000 pg/mL

Sensitivity: 4.89 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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